目的 研究土木香内酯(alantolactone,ALT)对骨肉瘤细胞株143B和saos-2的恶性增殖抑制作用。方法 采用CCK-8和EdU法检测ALT对143B和saos-2细胞增殖的影响;细胞划痕法、Transwell侵袭法分别检测ALT对143B和saos-2细胞的迁移、侵袭能力的影响,流式细胞术检测ALT对143B和saos-2细胞周期和凋亡的影响;qPCR和WB法分别检测ALT对143B和saos-2细胞中LAT1、SKA2、BUB1B、BCL2、STAT3mRNA和蛋白表达量的影响。结果 ALT能够抑制143B、saos-2细胞增殖、迁移和侵袭,将细胞阻滞于G1期,并促进细胞凋亡(P<0.05 或P<0.01)。与对照组相比,ALT作用后,143B、saos-2细胞中LAT1 mRNA和蛋白、SKA2 与BUL1B mRNA和蛋白水平显著下调,BCL2 与STAT3 mRNA和蛋白水平显著下调(P<0.05或P<0.01)。结论 ALT可抑制骨肉瘤143B和saos-2细胞的增殖迁移与侵袭,并将细胞阻滞于G1期,诱导其凋亡,其机制可能与抑制氨基酸转运(LAT1)、抑制细胞周期调控(SKA2、BULB1)、促进凋亡(STAT3、BCL2)等多环节有关。
Abstract
OBJECTIVE To investigate the inhibitory effect of alantolactone(ALT) on the proliferation of 143B and saos-2 cells of osteosarcoma cells. METHODS The proliferation and vitality effect of ALT on 143B and saos-2 cells were evaluated by CCK-8 and EdU assay. The invasion and migration effect of ALT on 143B and saos-2 cells were detected by cell invasion and cell scratch method. The effects of ALT on the cell cycle and apoptosis of 143B and saos-2 cells were detected by flow cytometry. The effects of ALT on LAT1, SKA2, BUB1B, BCL2 and STAT3 mRNA and protein levels in 143B and saos-2 cells were measured by qPCR and WB. RESULTS ALT could significantly inhibit the proliferation, migration and invasion of 143B and saos-2 cells. ALT could block cells at G1 phase, and induce the apoptosis of 143B and saos-2 cells(P<0.05 or P<0.01). Compared with the control group, the expressions of LAT1mRNA and protein, SKA2 and BUB1BmRNA and protein, BCL2 and STAT3mRNA and protein in 143B and saos-2 cells were all decreased by ALT, and the difference was statistically significant(P<0.05 or P<0.01). CONCLUSION ALT can inhibit the proliferation, migration and invasion of osteosarcoma 143B and saos-2 cells, and induce apoptosis and arrest the cell cycle of 143B and saos-2 in G1 phase, and its mechanism may be related to multiple links such as inhibiting amino-acid transport(LAT1),regulating cell cycle(SKA2,BUB1B), and promoting cell apoptosis(STAT3,BCL2).
关键词
骨肉瘤 /
土木香内酯 /
迁移 /
侵袭 /
增殖 /
凋亡
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Key words
osteosarcoma /
alantolactone /
migration /
invasion /
proliferation /
apoptosis
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中图分类号：
R965
R285.5
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脚注
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基金
公立医院高质量发展试点中央补助2022(个体化药物治疗实验室建设)项目资助(ZX-2208-2)
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